Genomics Support Services

To ensure a successful sequencing run, the sample must meet sufficient purity, quantity, and quality standards. Your submitted samples will be checked to meet these standards, and this QC assessment is included in the library prep price for each submitted sample that goes on to be prepared for sequencing. If the submitted sample needs to be improved, however, we are happy to work with the investigator to reach the necessary standards. Any additional QC assessment will be charged according to the current posted pricing sheet. 

 
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nanodrop one spectrophotometer

Designed to work specifically with downstream applications such as NGS projects, the NanoDrop One assesses sample contaminants and reports the adjusted sample quantity accordingly.

Qubit 3.0 fluorometer

The Qubit has the ability to differentiate between dsDNA, ssDNA, RNA, and protein. Concentration is equal to this signal of fluorescent dye emitted only when the dye binds to the sample being assayed. Most contaminants have no effect on the final result. This makes the Qubit extremely useful when accurate quantitation is needed for downstream applications.

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Agilent 2100 Bioanalyzer system

The Agilent 2100 Bioanalyzer system analyzes total RNA, small and micro RNAs, and varying sizes of DNA fragments. This chip-based capillary electrophoresis has a low sample consumption of 1µL providing the GC with an effective method of determining fragment size distributions. However, the Bioanalyzer is not equipped for accurate quantification of concentration.This device serves as the indicator of whether a sample can continue for further sequencing.

 

agilent 4200 tapestation system

The Agilent 4200 TapeStation system is fully automated and designed specifically to support Next Generation Sequencing workflows. Many sample types can be analyzed on the TapeStation, including total RNA, labeled RNA, small and micro RNAs, as well as small and large DNA fragments.

The most notable improvement this technology brings to our facility is its scalability. Even when investigating the quality of a single sample, the TapeStation allows for a constant cost. No longer does a lab have to waste funds because it is forced to pay in 12 sample increments.

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